Please use our optimized protocols for DNA extraction and sample preparation for amplicon sequencing for reproducible results that can be compared across different studies.

DNA extraction from anaerobic digester sludge

DNA extraction from influent wastewater

DNA extraction from activated sludge

MiSeq Sequencing of Amplicons

Barcodes and primers

16S rRNA V1-3 Amplicon Preparation


Read more about molecular methods in wastewater treatment in:

Chapter 8: Molecular Methods, Karst, S.M., Albertsen, M., Kirkegaard, R.H., Dueholm, M.S.,Nielsen, P.H.

Experimental methods in wastewater treatment, Loosdrecht, M.C.M. van, Nielsen, P.H., Lopez-Vazquez, C.M., Brdjanovic, D., IWA Publishing (Eds.), 2016. First published. ed. IWA Publishing, London.

You can download the book here.



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